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96 well plates  (Beyotime)


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    Structured Review

    Beyotime 96 well plates
    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of <t>a</t> <t>96-well</t> plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images
    96 Well Plates, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/black+opaque+plates/pmc13077891-125-14-22?v=Beyotime
    Average 93 stars, based on 34 article reviews
    96 well plates - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types"

    Article Title: A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types

    Journal: Cancer Cell International

    doi: 10.1186/s12935-026-04250-1

    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images
    Figure Legend Snippet: The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Techniques Used: Suspension, Transferring, Injection



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    Image Search Results


    The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Journal: Cancer Cell International

    Article Title: A new inject-embed 3D culture method enables the spheroid and aggregate formation from single or dual liver cancer cell types

    doi: 10.1186/s12935-026-04250-1

    Figure Lengend Snippet: The newly established inject-embed 3D culture method allowed the spheroid formation of all tested cells. ( A ) Schematic diagram of the inject-embed 3D culture method. The process includes three steps, i.e., prepare 100 µl of Matrigel diluted 1:1 with culture medium in each well of a 96-well plate; prepare a cell suspension; inject 10 µl of the cell suspension into the solidified gel with a pipette. Then 100 µl of culture medium was added to each well, and replace the medium as required. ( B ) 3D culture of HUCCT1, HLF, and Huh7 cell lines using the inject-embed method, traditional mix-embed method, and hanging drop method. An initial cell number of 1,000 was used for each cell line. ( C ) The sizes and morphologies of spheroids from seven cell lines under the inject-embed 3D culture. HCC, hepatocellular carcinoma. CCA, cholangiocarcinoma. TME, tumor microenvironment. An initial cell number of 4,000 per well in a 96-well plate was used for each cell line. The images were captured 4 days after injection, and scale bars are shown in the images

    Article Snippet: In 2D culture, 4,000 Huh7 and 2,000 HLF cells per well were seeded in 96-well plates (BeyoGoldTM black 96-well plates, Cat# FCP966-80pcs, Beyotime).

    Techniques: Suspension, Transferring, Injection